Ocean Optics Transmissive pH Probe - Manual (Page 4)

pH Probe Instructions
4 PH-BCG-TRANS-01-0210
Installing the pH Sensor System
► Procedure
Perform the steps below to install the pH Sensor components:
1. Install SpectraSuite software on your computer.
2. Connect the spectrometer to your computer using the supplied USB cable.
3. Install the light source as specified in its instructions.
4. Attach the fibers between the spectrometer, probe, and light source. The transmissive probe
has two identical legs.
5. Turn on the light source and allow it to warm up for the period specified in the light source
instructions.
6. The cap for the transmissive probe screws off. The probe uses a thin sensing film with
adhesive on one side. This can be affixed to the lens of the probe. A second patch can be
affixed to the mirror as well to dramatically increase signal strength and pH resolution.
Calibrating the pH Sensor System
The pH sensor patches include a pre-calibrated pK value determined at the factory. This value was
originally obtained at 22°C, and it is recalculated using the temperature compensation algorithm based
on the temperature that was entered in SpectraSuite’s Calibration Wizard. Using the Factory
Calibration method is ideal for being able to start making pH measurements quickly, though it is less
accurate than performing an Independent Calibration. The specifications listed for the probes assume a
complete Independent Calibration, as this eliminates the errors seen from temperature and other
environmental differences.
Using Factory Calibration
► Procedure
1. Open SpectraSuite and select File | New | New Sol Gel pH Measurement.
2. Click the Calibration Wizard button to begin the calibration.
3. Select the spectrometer to use and click Next.
4. Select Use Factory Calibration. Click the box for Using Reflective Probe, and then click
Next. The Enter Experimental Parameters screen appears.