Hoefer SE615 - Manual (Page 4)

Introduction
Gel sizes
The Hoefer ® SE615 and SE675 Multiple Gel
Casters are designed for casting vertical slab
acrylamide gels of homogenous or gradient
concentration.
Use the casters with 16 × 18 cm glass plates and
2 cm-wide spacers to cast 16 × 14 cm gels. Use
1-cm wide spacers to cast 16 × 16 cm gels for
two-dimensional electrophoresis. The resulting
gels are compatible with Hoefer SE400, SE600
and SE600 Chroma Vertical Slab Gel Electro-
phoresis Units.
Homogeneous and gradient gels
The SE615 and SE675 can be used for casting
both homogeneous and gradient gels. Gradient
gels are cast through the port at the bottom of
the caster. When casting homogeneous gels, the
results are most consistent when the gels are cast
from the bottom. The V-shaped region at the
bottom of the casting unit allows the gel solu-
tion to rise evenly, producing identical gels.
•
p1