Hoefer SE615 - Manual (Page 20)

Removing polymerized gels
1
After the gels have polymerized, place the caster in a
horizontal position. If you poured stacking gels, leave
the combs in place. Remove the faceplate.
2
Tip the caster to pour off the glycerol solution.
3
Slide the stack of sandwiches, acrylic blocks, and
polycarbonate sheets out of the caster. Remove the
acrylic blocks and polycarbonate sheets from the top
of the stack.
4
Carefully remove individual glass and gel sandwiches
from the stack. If necessary, insert a Wonder Wedge
between adjacent sandwiches to separate them.
Work slowly and cautiously when prying apart adjacent
sandwiches after polymerization.
5
Rinse the caster and polycarbonate sheets, as
described in “Care and maintenance” on page 20.
To store gels for future use
Rinse the individual sandwiches with distilled water.
Fill cassettes with gel storage buffer. Wrap gel sand-
wiches you are not using at this time in plastic wrap
and store them in the refrigerator.
To use gels immediately
1
Gently remove the combs by pulling them upward and
out of the gel sandwich.
2
Fill the wells with SDS Electrophoresis buffer.
3
Load samples and complete assembly. Refer to the
user manuals that come with SE600 or SE400 Series
electrophoresis systems.
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p17
Note: The SDS buffer makes
the glass plates slippery.
Note: Do not use metal
spatulas to separate the
sandwiches. Narrow metal
spatulas often chip the edges of
glass plates, making the plate
ineffective for sealing into the
electrophoresis tank.
Note: Separate the sandwiches
from one another before placing
the gels in the refrigerator.