Hoefer SE615 - Manual (Page 19)

4
Replace the caster in a vertical position. Make sure
the surface of the separating gel is free of liquid.
Use a pipette to fill each sandwich individually with
stacking-gel monomer solution.
5
Hold a comb, attached to a comb back, at a 45°
angle and centered within the sandwich. Insert one
end into the gel so that the end tooth is almost
completely inserted. Slowly lower the remaining comb
teeth in one by one, rotating the comb gradually
downward until it is in a horizontal position.
Refer to Fig 1 on page 4 for the final position of the
comb. Repeat this for each sandwich.
6
Allow the stacking gels to polymerize for at least one
hour. To remove the polymerized gels, see “Removing
polymerized gels” on page 17.
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p16
Note: Oxygen inhibits gel
polymerization. Do not trap
air bubbles underneath the
comb teeth.
Note: Use gels with stacking
gels immediately. Do not store.