Hoefer SE615 - Manual (Page 13)

Caution! Isobutanol crazes
or clouds the acrylic caster
walls, making it difficult to see
the gels.
2
Add initiator and catalyst, and pour the solution into
the glass sandwiches from the top.
The solution flows from one sandwich to another
through the groove at the bottom of the caster and
rises to the same level in all sandwiches. Pour slowly
to avoid overfilling.
3
After reaching the final level, overlay each gel
sandwich with 300 µl of water-saturated n-butanol
or buffer.
Use the same amount of overlay on each gel sandwich
to assure that all the gels polymerize to the same
heights. Pour the liquid gently, taking care not to
disrupt the gel surface.
After polymerization is complete, remove the
n-butanol and replace it with buffer or add stacking
gel. Water-saturated n-butanol will cloud acrylic parts
if used in large amounts or for long periods.
4
Allow the gels to polymerize for at least an hour. To
remove the polymerized gels, see “Removing polym-
erized gels” on page 17. To add a stacking gel, see
“Pouring stacking gels” on page 15.
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