Appendix a: gel identification numbers, Appendix b: references – Hoefer SE615 User Manual
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Appendix A: Gel identification
numbers
For positive identification of gels, label each slab
by incorporating a small label printed on thin
filter paper in the bottom corner of the gel. Use
a carbon typewriter ribbon, photocopier or laser
printer to make these labels, since many liquid-
based inks are electrophoresed off paper during
an SDS electrophoresis run.
A variety of numbering schemes are possible.
In our experience the easiest uses three parts
as follows:
• An upper-case letter to identify the investigator
or an extended gel series.
• A two- or three-digit serial number to identify
the slab gel batch.
• A lower-case letter to identify a gel in the
batch. Since a maximum of 14 gels can be
made in a batch, use the letters a–n.
The resulting numbers, in the format A63a,
A63b...., etc., provide a useful system for keep-
ing track of and cross-indexing experiments and
gel production.
Appendix B: References
Laemmli, U.K. 1979. Nature (London) 227:
680–685.
SDS-PAGE and IEF Handbook.
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