Labconco Mini Stoppering Chamber - Manual (Page 3)

Labconco Instruction Sheet 1049200, Rev -, ECO H250 01/13 3 of 5
Freeze Dry
1. The samples should be pre-frozen in the vials with the stoppers installed in the un-
stoppered position. A tray is provided so that the vials can be loaded and then placed in
the freezer. Once the samples are frozen and the Freeze Dryer is ready, the tray can
quickly be transferred to the Small Stoppering Chamber for Lyophilizing.
2. Start the Freeze Dry System as directed in the owner’s manual. When the Freeze Dry
system is started all of the Freeze Dry Ports should be in the vent position. (The bevel on
the knob should point away from the sample port). This will allow vacuum to build up in
the system.
3. When the freeze dry system is ready, make sure the switch on the Small Stoppering
Chamber in the “CLOSE” position. Place the tray with the frozen samples in the Small
Stoppering Chamber with the proper spacers. Make sure the Clear Chamber and Clear
Lid are centered and resting on their o-ring seals. Apply vacuum to the sample by rotating
the knob on the Freeze Dry Port to the vacuum position. (The bevel on the knob should
be positioned toward the sample port).
4. The vacuum should pull the Clear Chamber and Clear Lid together to seal the system.
Check the Vacuum after some time to confirm there are no vacuum leaks.
5. Lyophilization has begun.
Stoppering
1. Let the samples run until primary and secondary freeze-drying is complete.
2. To stopper the vials move the toggle switch on the Small Stoppering Chamber to the
middle position marked “STOPPER”. The vials will move up, the stoppers will press
against the clear lid and then seal the vials.
3. Once all of the vials are stoppered return the toggle switch to the closed position.
4. Release vacuum by turning the Freeze Dry Port to the vent position.
Notes:
Always vent the vacuum line before turning off the vacuum pump.
Make sure all vacuum lines are secured to prevent leaks.
Wear appropriate protective attire and eye protection.
Clean after use.
Installation of Small Chamber Cold Trap (Refer to Figure 2)
6. Place one end of the PVC Tubing over the ½ fitting on the side of the chamber and
secure with a clamp.
7. Insert the ½” Stainless Steel Adapter approximately 1” into the other end of the PVC
Tubing and secure with a clamp.
8. Insert the open end of the ½” adapter into one of the Ports on the Freeze Dryers
Chamber.