ChemoMetec SCC-400 - Manual (Page 15)

Table!of!contents!
1!
Table!of!contents!
DECLARATION!OF!CONFORMITY!.....................................................................................!VII
!
WEEE!DIRECTIVE!INFORMATION!–!EUROPE!ONLY!..............................................................!IX !
INTRODUCTION!AND!INTENDED!USE!................................................................................!XI !
WARNINGS!AND!PRECAUTIONS!......................................................................................!XIII !
1 ! INSPECTION,!UNPACKING!AND!INSTALLATION!OF!EQUIPMENT!.....................................!3 !
2
!
CELL!COUNTING!WITH!THE!NUCLEOCOUNTER!SCC0400!.................................................!5
!
2.1
!
T HE! N UCLEO C OUNTER! SCC0400 !INSTRUMENT !.................................................................!5
!
2.1.1 ! Fluorescence%microscope%................................................................................%5 !
2.1.2
!
The%Sample%Compartment%fixture%...................................................................%5
!
2.1.3
!
Sample%Intake%................................................................................................%6
!
2.1.4 ! The%RUN%Key%and%LED%indicator%......................................................................%6 !
2.1.5
!
Internal%Flow%System%.....................................................................................%6
!
2.2 ! S AMPLE! I NTAKE !.......................................................................................................!6 !
2.2.1
!
Introducing%Samples%......................................................................................%6
!
2.2.2 ! Sample%Compartment%Cleaning%.......................................................................%7 !
3
!
START0UP!..................................................................................................................!8
!
3.1 ! P OWER!ON , !POWER!OFF !.............................................................................................!8 !
3.1.1 ! Starting%up%....................................................................................................%9 !
4 ! RUN!BUTTON,!LED!INDICATOR!AND!PC!CONTROL!.........................................................!9 !
4.1
!
I NSTRUMENT! S TATUS !..............................................................................................!10
!
4.2 ! I NTERACTIVE! C ONTROLS !...........................................................................................!10 !
4.2.1
!
Analyze%Sample%...........................................................................................%10
!
4.2.2 ! Rinse%of%Flow%System%...................................................................................%10 !
4.2.3
!
Clean%of%Flow%System%...................................................................................%10
!
5
!
OPERATION!OF!THE!NUCLEOCOUNTER!......................................................................!12
!
5.1 ! S TART! N UCLEO V IEW! SCC0400 ! A PPLICATION !.................................................................!12 !
5.2 ! N UCLEO V IEW! SCC0400 ! W INDOW !..............................................................................!12 !
5.3 ! N UCLEO V IEW! SCC0400 ! B UTTONS !..............................................................................!13 !
5.3.1 ! Run%Button%..................................................................................................%13 !
5.3.2 ! Rinse%Button%................................................................................................%13 !
5.3.3
!
Clean%Button%...............................................................................................%14
!
5.3.4 ! Barcode%Button%............................................................................................%14 !
6 ! NUCLEOVIEW!SCC0400!..............................................................................................!15 !
6.1.1
!
Sample%Image%..............................................................................................%15
!
6.1.2 ! Object%Intensity%Histogram%...........................................................................%16 !
6.2 ! N UCLEO V IEW! SCC0400 ! M ENU! F UNCTIONS !..................................................................!16 !
6.2.1 ! File%U%Load%Result%File%...................................................................................%17 !