ANKOM RF - Manual (Page 39)

Operator’s Manual
Rev F 8/29/14
pg. 39
10. Add Sample, Buffer, and Inoculum to all non-Blank Glass Bottles to be used in your study.
Place your sample, buffer, and inoculum into the non-Blank Glass Bottles.
11. Eliminate the oxygen from the non-Blank Glass Bottles.
If you are running a study that includes anaerobic organisms, then eliminate the oxygen from the non-Blank
Glass Bottles by following the procedure detailed in step 9 above.
12. Configure your GPM software for your study.
12.1 On your GPM screen, set the Live Interval duration. This is the number of seconds that the
system will wait between each Module-to-computer communication. Longer live interval times
increase battery life.
12.2 On your GPM screen, set the Recording Interval. This is the number of minutes the system will
wait between each data point that gets recorded to the GPM spreadsheet for each Module.
For best results it is recommended that the Live Interval be set so that there are
at least 2 Live Intervals for every Recording Interval. For example, if you want
to record data every 2 minutes, you should set the Recording Interval to 2
minutes and the Live Interval to 60 seconds or less.
12.3 On your GPM screen, set the Pressure Units.
12.4 On your GPM screen, set the Pressure Release value for each Module. This is the pressure that
when reached will initiate the opening of the Module’s valve during the live period.
12.5 On your GPM screen, set the Autosave location file name to establish where you want the
autosave process to store your data.
13. Place the Module Assemblies in an incubator or water bath set to the appropriate temperature.
Do not allow water into the Module: When placing a Module Assembly in
water, do NOT allow the water level to reach the vent tube or damage will occur.
When using a water bath, do NOT cover the bath and the RF modules with a lid
as the trapped humidity will be detrimental to the electronics. Instead, use bath
balls to cover the surface of the water and control the temperature and
evaporation.
14. Start recording data.
Before you start recording data, replace batteries that show 6.6 volts or lower.
On your GPM screen, click Record to start recording your data.
15. Stop recording data at the end of the study.
On your GPM screen, click Stop to stop recording data. An Excel spreadsheet will be created with your data
once you enter a file name.
If you want to create an Excel file during a run, just press the Save… button on
the GPM screen. The program will continue to run as normal.