ANKOM RF - Manual (Page 38)

Operator’s Manual
pg. 38
Rev F 8/29/14
9. Eliminate the oxygen from the Glass Bottle used as a Blank (i.e., purge the bottle).
If you are running a study that includes anaerobic organisms, then eliminate the oxygen from the Glass Bottle
used as a Blank by following the procedure below.
You will need a source of CO
2
regulated to 8-10 psi for this procedure.
9.1 After adding inoculum to the Glass Bottle and with it in position to reattach to its Module (when
using wide-mouth bottles with metal clip closures leave the lid slightly open so that it can be
quickly closed), add CO
2
directly into the top of the bottle until the CO
2
fills the Module’s Glass
Bottle. This could take about 5 to 30 seconds depending upon the size of the bottle.
9.2 Immediately reattach the Module to the Glass Bottle.
9.3 On your GPM screen, set the Live Interval to 1 sec to speed up the valve open and close
operation.
9.4 On your GPM screen, set the Global release to 8 psi and set the Valve open time to 1000 ms.
9.5 Remove the cap from the Luer Port on the Module.
9.6 Holding a CO
2
supply against the Luer Port, add 8-10 psi of CO
2
to the Module’s Glass Bottle
(see below). When the pressure exceeds 8 psi the valve will open and begin to release gas.
Allow the bottle to sit for 10 seconds allowing time for the gases to equilibrate. Set the
Global
release to zero, releasing all pressure within the Glass Bottle. Ensure the pressure in the Glass
Bottle is back to 0

0.2 psi. Reset the Global release setting to 8 psi and repeat this step two
more times to thoroughly purge undesired gases from the Glass Bottle.
9.7 Remove / turn off the CO
2
supply and place the cap on the Luer Port.
9.8 Repeat this procedure for each Module.
9.9 Reset the Live Interval, Valve open time, and Global release settings on the GPM screen based
on the needs of the experiment.
WARNING: For 250 ml – 1000 ml narrow-mouth bottles, never allow the
pressure in the bottles to exceed 10 psi. Always regulate incoming purge
pressure down to 10 psi or less before purging bottles. For 1.8 L wide-mouth
bottles, never allow the pressure in the bottles to exceed 1 psi. Always wear
safety glasses and appropriate lab protection when handling the Modules and
Glass Bottles.
Holding the vent valve open may reduce battery voltage. Before clicking the
Record button on your GPM screen to start a study, replace batteries that show
6.6 volts or lower.
Luer Port
Purge Air Gun
(part# RF28 – sold separately)