Bio-Rad Nuvia™ cPrime™ Media - Manual (Page 8)

5
Flow Properties
Fig. 1. Nuvia cPrime media pressure vs. flow performance for a
20 cm diameter column and a 20 cm bed height; compression ratio
1.17. Nuvia cPrime media have fast mass transfer properties allowing
users to achieve high productivity at fast flow rates. The media should
be run at the highest linear velocities that allow good separation and are
allowed by the column and chromatography system specifications. A
linear flow rate of 300 cm/hr and a 20 cm bed is a recommended starting
point.
Fig. 2. Effect of flow rate on Nuvia cPrime media binding capacity for
lactoferrin.
Column dimension: 1.1 x 9.6 cm
Sample: 5.25 mg/ml lactoferrin
Nuvia cPrime Media vs. Pressure Flow
-0.5
0
0.5
1
1.5
2
2.5
3
0 200 400 600 800 1000 1200
Linear flow rate, cm/hr
Pr
es
su
re
, b
ar
Nuvia cPrime Media DBC (Lactoferrin) vs. Flow Rate
0
10
20
30
40
50
60
70
80
150 300 450 600
Flow rate, cm/h
10
%
B
T
DB
C,
m
g/
m
l