Bio-Rad Nuvia™ cPrime™ Media - Manual (Page 11)

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Section 6
Method Development
Developing an effective and robust method with Nuvia™ cPrime™
media is straightforward. Below is general information on the
binding and elution mechanism and an approach to guide method
development; results will vary depending on protein of interest and
feed composition.
The binding and elution mechanisms of Nuvia cPrime media
are determined chiefly by pH and salt. The high salt tolerance of
the media often allows for direct loading at high conductivity. An
increase in pH will in most cases achieve elution. Conductivity
is another way to achieve and/or optimize elution and the final
method is often a combination of an increase in pH and/or an
increase/decrease in salt concentration. In some cases, the use of
an elution buffer modifier or a different salt in the elution buffer may
be required for optimal elution, recovery, and resolution.
The schematic below outlines a general method development
rational. In most cases, conducting a few simple DOE experiments
to identify optimal binding and elution conditions will yield an
effective, robust, and scalable method.