Bio-Rad Nuvia™ cPrime™ Media - Manual (Page 6)

3
Section 3
Preparation
Nuvia™ cPrime™ media are supplied fully hydrated in 20% ethanol
as a 50% (v/v) slurry. For column packing, removal of the shipping
buffer is recommended. Small volumes of Nuvia cPrime media are
easily washed in a Büchner funnel with 4–5 volumes of packing
buffer. For large volume preparation, cycle through 3–4 settling and
decanting steps using the column packing buffer in the shipping
container.
Removal of fines from Nuvia cPrime media is not required. If, however,
particle fines have been generated during handling, resuspend the settled
media and remove any opaque or cloudy supernatant before resettling is
complete. Repeat several times until supernatant is clear.
Section 4
Column Packing
Nuvia™ cPrime™ media can be packed using standard column
packing methods. To pack columns for optimal operation, a
20–50% slurry volume is recommended.
Packing Small Columns
This slurry packing method was designed to pack Nuvia cPrime
media in a conventional laboratory scale column with an internal
diameter of 5–50 mm. All buffers should be degassed. Since a
relatively large volume of slurry is required, a packing reservoir
should be used.
1. Prepare degassed 1.0 M NaCl, 20–50 mM buffer salt (see
Table 2) referred to herein as the packing buffer.
2. Decant the shipping solution away from the resin bed as
outlined in Section 3, maintaining an approximate slurry
percentage of 50%.
3. After thorough buffer exchange, prepare an aliquot of Nuvia
cPrime media in a graduated cylinder to determine the slurry
percentage.
4. Seal the cylinder and rotate it to suspend the resin. Caution:
do not mix with a magnetic stir bar as damage may occur.
Larger amounts of slurry may be mixed with a low-shear
impeller at low to moderate speed.