Bio-Rad Nuvia™ S Media - Manual (Page 8)

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Table 2. Nuvia S media specifications.
Type of exchanger Strong cation
Functional group –SO
3
–
Total ionic capacity 90–150 µeq/ml
Dynamic binding capacity * ≥110 mg/ml (300 cm/hr)
Shipping counterion Na +
Median particle size 85 ± 15 µm
Recommended linear flow rate ** 50–600 cm/hr
Chemical stability
1.0 N NaOH (20°C) Up to 1 week
0.1 N NaOH (20°C) *** Up to 5 yr
pH stability *** 2–14 short-term
4–13 long-term
Shipping 20% ethanol + 0.1 M NaCl
Regeneration 1–2 M NaCl
Sanitation 0.5–1.0 N NaOH
Storage 20% ethanol or 0.1 N NaOH
* 10% breakthrough capacity determined with 4.5 mg/ml human IgG in
40 mM NaOAc, 30 mM NaCl, pH 5.0.
** Elution flow rates <150 cm/hr minimize viscosity-induced backpressure.
*** Data derived under accelerated conditions at 60ºC.