Bio-Rad Nuvia™ S Media - Manual (Page 21)

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At the end of each run, the cartridge can
be regenerated with 1.0 M NaCl followed by
starting buffer. Return to the desired flow rate
and proceed with the next separation.
4.3 Purification Scale-Up
For quick scale-up, two or three cartridges
of the same type can be connected in series.
Backpressure will increase with cartridges in
series, so care should be taken to maintain
pressures ≤45 psi. Bio-Scale Mini cartridges
are available in 1 and 5 ml cartridge formats.
Nuvia S support is also available in larger
amounts, from 25 ml bottles to bulk quantities
for scaling-up methods developed using
the cartridges. Nuvia S media are fully
supported with regulatory support files. In
addition, Bio-Rad carries an extensive line of
empty chromatography columns for use in
laboratory and process scale applications.