Bio-Rad Nuvia™ S Media - Manual (Page 19)

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Table 3. Products for buffer exchange.
Sample
Volume
Product MW Cutoff Catalog #
50–100 µl Bio-Spin ® 6 6 kD 732-6002
columns
Bio-Spin 30 30 kD 732-6006
columns
0.1–3 ml Bio-Scale 6 kD 732-5304
Mini P6 cartridges
Up to 3 ml Econo-Pac ® 10DG 6 kD 732-2010
cartridges
Unlimited Bio-Gel ® P-6DG gel 6 kD 150-0738
4.2 General Purification Protocol
Ion exchange chromatography is usually
performed using increasing salt gradients
or pH gradients to elute the sample
components. For best results and increased
cartridge life, samples and buffers should be
degassed and filtered through a 0.45 µm filter.
Common buffers are listed in Table 4.