Bio-Rad Nuvia™ S Media - Manual (Page 17)

13
Section 4
Preparing a Cartridge for
Use
Bio-Scale ™ Mini Nuvia ™ cartridges contain
20% ethanol + 0.1 M NaCl (v/v) as the
shipping solution. The fully hydrated support
is ready to use after equilibrating the cartridge
in the buffer of choice. To perform a buffer
exchange, connect the cartridge to a liquid
chromatography system or peristaltic pump
and condition it as follows:
1. Set the pump flow rate to 1–2 ml/min
(240–280 cm/hr) for the 1 ml cartridge or
5–10 ml/min (140–480 cm/hr) for the 5 ml
cartridge.
2. Wash the cartridge with degassed low-salt
buffer for 2 min.