Bio-Rad VersaFluor™ Fluorometer - Manual (Page 25)

Section 8
Filters for Fluorescent Protocols
Excitation Catalog Emission Catalog
Application Filters Number Filters Number
DNA Quant (Hoechst 33258) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
ß-Galactosidase (MUG) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
ß-Glucuronidase (MUGluc) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
Apopain (Z-DEVD-AFC) EX 360/40 (340–380 nm) 170-2420 EM 520/10 (515–525 nm) 170-2424
pH indicator (BCECF) EX 490/10 (485–495 nm) 170-2422 EM 520/10 (515–525 nm) 170-2424
Calcium indicator (Fluo-3) EX 490/10 (485–495 nm) 170-2422 EM 520/10 (515–525 nm) 170-2424
DNA Quant (PicoGreen™) EX 480/20 (470–490 nm) 170-2427 EM 520/10 (515–525 nm) 170-2424
Oligo Quant (OliGreen™) EX 480/20 (470–490 nm) 170-2427 EM 520/10 (515–525 nm) 170-2424
Protein Quant (NanoOrange™) EX 480/20 (470–490 nm) 170-2427 EM 590/10 (585–595 nm) 170-2425
Fluorescein EX 490/10 (485–495 nm) 170-2422 EM 520/10 (515–525 nm) 170-2424
Ethidium Bromide EX 510/10 (505–515 nm) 170-2423 EM 620/10 (615–625 nm) 170-2426
RNA Quant (RiboGreen™) EX 490/10 (485–495 nm) 170-2422 EM 520/10 (515–525 nm) 170-2424
Section 9
Specifications
Instrument
Light source 20 watts incandescent quartz halogen 350 nm to 900 nm.
The quartz halogen lamp is rated to operate for 2000 hours.
Sensing device Photodiode
Optical filters Excitation 1” diameter with holder
Emission 1” diameter with holder
Sample source Cuvette: 12.5 mm x 12.5 mm (outside dimension)
Sensitivity selection Three settings: LOW, MED, and HIGH.
Approximate factor of 10 between each setting.
Dynamic range Greater than 5 orders using all three sensitivity selec-
tions.
Printer output RS-232, Serial
AC Power Requirement
AC power input 100–240 VAC 50–60 Hz, 1.6 A slow-blow fuse
Size and Weight
Overall size 23 cm (L) x 33 cm (W) x 15 cm (H)
Shipping weight 4 kg
Environmental Requirements
Storage environment 0 to 70 °C, humidity 0–95% (non-condensing)
Operating environment 20 to 35 °C, humidity 0–95%
Regulatory
Meets the requirements of: EN61010-1, EN50082-1, EN55011/03
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