Bio-Rad VersaFluor™ Fluorometer - Manual (Page 21)

Section 6
Troubleshooting
6.1 Troubleshooting Table
Problem Cause Solution
No display with power on Burned out fuse Replace fuse.
No RFU values with samples Lamp OFF Insure that the lamp is ON.
Lamp burned out Replace lamp.
Thermal fuse tripped Call Bio-Rad Service.
Lamp OFF with power ON Lamp OFF Insure that the lamp is ON.
Thermal fuse tripped Call Bio-Rad Service.
Negative values Readings below set 1. Zero the instrument with the
zero (blank) range set to 00000. Set the range
with the highest concentration
standard.
2. Zero between gain settings.
Readings fluctuate Interference in light 1. Check that there are no scratches
path on the cuvette.
2. Check that there are no particu-
lates floating in the cuvette.
3. Wipe the cuvette walls with a lint-
free tissue.
4. The instrument reading may
fluctuate somewhat at high gain
setting with low concentration
samples.
5. Insure that the gain and range
have been set properly (Sections
4.3 and 4.4).
6.2 Performance Check—Internal Diagnostics
Under normal conditions, the VersaFluor Fluorometer will operate for many years with
minimal maintenance. If the instrument is operating, but a problem is suspected, run the per-
formance check.
1. The VersaFluor Fluorometer instrument can be tested by internal diagnostics which ver-
ify correct operation of the electronics. With the sample compartment door closed, the
internal diagnostics are accessed by holding down any of the four buttons while turning
on the instrument. The diagnostic display below will be shown.
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DIAGNOSTICS
***** MED .06FS LAMP