Bio-Rad Bio-Beads S-X Media - Manual (Page 9)

will be used during the separation. (This is not necessary for
Bio-Beads S-X1 beads.) Maintain at least a few centimeters of
liquid above the resin bed.
Never allow the packed beads to become dry, because this
will cause air pockets and channeling within the bed, resulting
in poor efficiency and low resolution. If a small column does go
dry, add extra solvent, cap both ends, invert several times until
the resin is fully slurried, allow the resin bed to settle, and begin
flow as normal. An alternative technique, for larger columns, is
to backwash the resin. This will resuspend the beads and allow
repacking. This technique should only be used if the packed bed
fills less than half the column. Simply connect a piece of tubing
to the column outlet, and, at a very slow flow rate, flow the elu-
tion solvent into the column. This will resuspend the packing
material. Care must be taken that enough space is available for
the resuspended material, as well as the additional solvent.
When the material is fully suspended, and the air pockets have
risen above the packing material, stop the flow. Allow the mate-
rial to settle.
Note: Bio-Beads S-X beads will float in high density solvents,
e.g. chloroform. First swell the media in tetrahydrofuran and
pack the column as previously described. Insert an adjustable
flow adaptor into the column to constrain the beads in a packed
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