Bio-Rad Bio-Beads S-X Media - Manual (Page 11)

matogram to establish the time periods to use when collecting a
specific fraction.
Regeneration
Regeneration of Bio-Beads S-X beads may be necessary if
compounds have become trapped within the pores of the resin,
for example if a series of eluants has been used. Bio-Beads S-X
beads are hydrophobic, and can also absorb compounds. To
wash the resin, swell it to its maximum with a solvent such as
methylene chloride, toluene, or tetrahydrofuran.
After long periods of storage, small loose polystyrene
which had been trapped inside the beads will leach out. This
polystryrene (2-10 chain length) has a very high extinction coef-
ficient, so even trace amounts will have a high UV absorption.
To eliminate this problem, wash with several bed volumes of
solvent.
Chemical Resistance
Bio-Beads S-X beads are highly chemically resistant,
though explosive mixtures may form in the presence of strong
oxidizing reagents such as chromic acid, nitric acid, and hydro-
gen peroxide. The beads may be autoclaved.
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