Bio-Rad Bio-Beads S-X Media - Manual (Page 8)

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Put together a clean column assembly. Place a small
amount of elution solvent in the column to prevent bubble
formation at the base of the poured column packing. Prepare
a solvent reservoir, which should be placed at an elevation
higher than the top of the column. The solvent reservoir may
be connected to the column by tubing. Any type of connec-
tion must be air-tight and clean.
The Bio-Beads S-X beads should be placed in an
approximately 50% slurry of beads and elution solvent. A
good practice at this point is to degas this mixture by soni-
cating it under vacuum. Safety precautions should be
observed. Double check that the stopcock below the column
is closed. Swirl the slurry to get a homogeneous mixture,
then slowly and consistently pour the slurry into the column.
It is best to pour a long narrow column in sections, in order
to obtain a uniform and reproducible column. A glass rod
may be used to facilitate pouring the mixture down the side
of the column. This will also help eliminate the formation of
bubbles. When it is clear that a few centimeters of packed
bed have settled, open the stopcock below the column and
allow the mobile phase to begin flowing. Begin with a slow
flow rate and gradually increase to 10-15% above the final
operating flow rate, if possible. The purpose of this elevated
flow rate is to pack the column under more pressure than
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