Bio-Rad Bio-Beads S-X Media - Manual (Page 5)

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Instructions for Use
Bio-Beads S-X beads are supplied dry, and must be
swollen prior to packing into a chromatographic column.
The general instructions are:
1. Swell the beads.
2. Assemble the column.
3. Pour the beads into the column.
4. Add the sample and proceed with the separation.
The instructions below describe swelling the beads and
packing the column in details.
Swelling the Beads
Before use, swell the Bio-Beads S-X beads in an organ-
ic solvent, such as:
Aromatics Methylene chloride
Benzene Orthodichlorobenzene
Carbon tetrachloride Perchloroethylene
Dimethylformamide Tetrahydrofuran
Ketones Trichlorobenzene
These organic solvents allow maximal swelling of the
beads. If polar solvents, such as water or methanol, are used,
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