Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 4)

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Table 1. Profinia purification and buffer kits.
Kit Catalog # Solutions Powdered Reagent Cartridges
Purification Desalting Storage
Buffers Buffer Solutions Glutathione Affinity Desalting
Profinia Native IMAC Kits
Native IMAC 620-0225
✔ ✔ ✔ ✔ ✔
purification kit 620-0235
✔ ✔ ✔ ✔ ✔
Native IMAC
buffer kit 620-0221
✔ ✔ ✔
Profinia GST Kits
GST purification 620-0226
✔ ✔ ✔ ✔ ✔ ✔
kit 620-0236
✔ ✔ ✔ ✔ ✔ ✔
GST buffer kit 620-0223
✔ ✔ ✔ ✔
Profinia Desalting Kits
Desalting kit 620-0228
✔ ✔ ✔
620-0238
✔ ✔ ✔
Desalting and 620-0224
✔ ✔
cartridge cleaning
buffer kit
1.3 Equipment and Materials Required
Profinia purification and buffer kits are specifically designed to be used
with the Profinia protein purification instrument. All of the buffers and
solutions come formulated as concentrates that are made with
chromatography-grade solutions, and have been prefiltered to remove
particulates. The buffers that require the addition of urea (not provided) or
glutathione require filtration through a 0.2 µm filter prior to use with the
system.
Accessories available for the Profinia instrument include Profinia
software, cooling blocks, and desalting sample loops, buffer bottles, and
tubing. Consumables that can be purchased separately include His- and
GST- detection antibodies, and a dual-tagged lyophilized control lysate
containing GST- and His-tagged proteins. Complete catalog and ordering
information can be found in Section 9.
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