Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 18)

Section 7
Frequently Asked Questions and
Troubleshooting
For an extensive and comprehensive list of troubleshooting tips for
protein expression, sample preparation, purification and analysis, refer to
the Profinia™ Protein Purification Instrument Instruction Manual.
Questions and tips below focus on the use of the buffer kits and solutions.
More information on Profinia applications can be found in the Profinia
FAQs on www.bio-rad.com.
Proper Storage of Solutions and Kits
• All of the kits can be stored at 22°C (room temperature) for short periods
of time, either upon receipt or during normal usage. For the kits that
have labile reagents (glutathione in GST kits), 4°C is the recommended
long-term storage temperature
• Once opened and used with the instrument, all reagents should be
stored at 4°C for up to a period of 3 months. After insertion into the
instrument, the solutions are no longer sterile and require 4°C storage
• Once opened and used with the instrument, check the solutions for
particulates and clarity before reusing. If there is any indication of
particulates, contamination, or microbial growth, the solution should
discarded
Addition of Additives or Reagents to the Solutions
• The solutions are provided as concentrates, and are diluted by the
preprogrammed methods in the instrument. If it is desired to add an
additional component to one of the solutions (such as, a protease
inhibitor), it should be added as a concentrate and brought to the final
concentration of the solution (such as, final 2x concentration for the
affinity buffers). The volume to be added should be minimized so that
it does not dramatically alter the final concentration of the solution
labeled on the bottle
Addition of Glutathione
• Urea (not provided) and glutathione should be added to the buffers on
the day purification starts. Any unused solution can be stored at 4°C
for up to 7 days
• For longer-term storage, any unused buffer can be stored at –20°C
and used within a 3 month period. Upon thawing, check for any
particulates, and if necessary, heat in a 37°C waterbath to dissolve
precipitates. Always refilter solutions through a 0.2 µm filter if
particulates are present
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