Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 13)

Bio-Gel ® P-6 size exclusion resin, and typically yield
≥
80% recovery of
applied target protein. The resin in the Bio-Gel P-6 desalting cartridges
exhibits minimal interaction with proteins. When used with the cleaning
buffers, different proteins can safely be applied to the same desalting
cartridge.
Table 5. Formulations for buffers and solutions provided in the
desalting kits.
Supplied
Profinia Solution As Concentrated Formulation Working 1x Formulation Volume Position #
Desalting buffer 5x 685 mM NaCl, 13.5 mM KCl, 137 mM NaCl, 2.7 mM KCl, 200 ml 4
21.5 mM Na
2
HP0
4
, 40.5 mM 4.3 mM Na
2
HP0
4
, 8.1 mM
KH
2
HP0
4
, pH 7.0 KH
2
P0
4
, pH 7.4
(pH 7.4 upon dilution)
Cleaning solution 1 2x 1,000 mM NaCl, 100 mM Tris, 500 mM NaCl, 50 mM Tris, 125 ml 5
pH 8.0 pH 8.0
Cleaning solution 2 4x 2,000 mM NaCl, 400 mM 500 mM NaCl, 100 mM 125 ml 6
NaOAc, pH 4.5 NaOAc, pH 4.5
Storage solution 2x 4% C
6
H
5
CH
2
OH 2% C
6
H
5
CH
2
OH 200 ml 7
(benzyl alcohol) (benzyl alcohol)
5.2 Instructions for Use
The buffers are provided as concentrates and are diluted to a 1x
formulation by the instrument using the preprogrammed methods. The
position labeling of the bottles and insertion into the instrument is identical
to that described in Section 2.2. Because desalting does not use any of
the affinity buffers, positions 1–3 are not used. Refer to Figure 5 for the
proper placement of the desalting buffer bottles. For desalting applications
using affinity purification as a first step, refer to Sections 2 and 4 — Native
IMAC Purification and Buffer Kits, and GST Purification and Buffer Kits.
Fig. 5. Desalting and cartridge cleaning buffer bottle positioning.
The desalting methods are designed for rapid desalting or buffer
exchange and require that a desalting loop accessory be used in
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