Bio-Rad Bio-Plex Pro™ Human Cancer Biomarker Assays - Manual (Page 26)

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4. Enter Standards Info into Bio-Plex Manager.
a. Enter the highest concentration of each analyte in the top row
(labeled S1) of the table. S1 concentration information is included
with each vial of standards.
b. Enter a dilution factor of 4 and click Calculate. The concentrations
for each standard point will be populated for all analytes in the table.
c. Optional: enter the lot number of the vial of standards into the
Standard Lot box and click Save.
Fig. 4. Plate formatting.