Bio-Rad Bio-Plex Pro™ Human Cancer Biomarker Assays - Manual (Page 25)

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3. Format the plate in Bio-Plex Manager according to the plate layout
created in section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 4).
a. Select the Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all
the wells that contain standards. Repeat this process for
Blanks
B
, Controls
C
, and Samples
X
. Note that
Bio-Plex Manager automatically subtracts the blank FI
value from all other assay wells.
Table 13. Bead regions for human cancer biomarker panels.
Panel 1
Analyte Bead Region Analyte Bead Region Analyte Bead Region
sEGFR 15 sIL-6R a 19 Prolactin 52
FGF-basic 44 Leptin 78 SCF 65
Follistatin 26 Osteopontin 77 TIE-2 64
G-CSF 57 PDGF-AB/BB 47 sVEGFR-1 76
sHER2/neu 12 PECAM-1 46 sVEGFR-2 45
HGF 62
Panel 2
Angiopoietin-2 47 IGFBP-1 62 TGF- a 76
sCD40L 29 IL-6 19 TNF- a 36
EGF 14 IL-8 54 uPA 78
Endoglin 26
IL-18 42 VEGF-A 45
sFASL 35 PAI-1 61 VEGF-C 64
HB-EGF 57 PLGF 52 VEGF-D 43