Bio-Rad Bio-Plex Pro™ Human Cancer Biomarker Assays - Manual (Page 24)

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Bio-Plex Manager software versions 6.0 and higher contain protocols for
most Bio-Plex
® assays. Choose from available protocols or create a new
protocol. To create a new protocol, select File, then New from the main
menu. Locate and follow the steps under Protocol Settings.
1. Describe Protocol and enter information about the assay (optional).
2. Select Analytes and create a new panel. Visually confirm the selected
analytes and proceed to step 3.
a. Click the Add Panel button in the Select Analytes toolbar.
Enter a new panel name. Select Bio-Plex Pro Assay Magnetic
from the assay dropdown list. If using Bio-Plex Manager version
5.0 or lower, select MagPlex from the assay dropdown list.
b. Click the Add button. Enter the bead region number and name
for the first analyte. Click Add Continue to repeat for each analyte
in the assay. Refer to the bead regions in parentheses ( ) listed on
the peel-off label provided with the standards.
For reference, bead regions for human cancer assays are shown
in Table 13.
c. Click the Add button when the last analyte has been added and
click OK to save the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click the Add All button.
e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking on Rename Panel and entering a
new panel name.