Bio-Rad Bio-Plex Pro™ Human Cancer Biomarker Assays - Manual (Page 15)

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6. Use reconstituted and diluted standards and controls immediately. Do
not freeze for future use.
Fig. 3. Preparing a fourfold dilution series with a single reconstituted standard.
5. Prepare Samples
General guidelines for preparing different sample types are provided
here. For more information, consult publications listed in Bio-Rad bulletin
#5297, available for download at www.bio-rad.com, or contact Bio-Rad
Technical Support.
n
Once thawed, keep samples on ice. Prepare dilutions just prior to the
start of the assay and equilibrate to room temperature before use
n
Do not freeze diluted samples
150 150 150 150 150 150 150 150
S1 S2 S3 S4 S5 S6 S7 S8 Blank
Diluent, µl
50 50 50 50 50 50 50 Transfer Volume, µl
Reconstituted
Standard
Table 5. Summary of recommended sample diluents and dilution factors.
Sample Type
Diluent Add BSA Sample Dilution*
Serum and plasma Sample diluent HB None Fourfold (1:4)
Culture media,
with serum Culture media None User optimized (neat to 1:10)
Culture media,
serum-free Culture media To 0.5% final* User optimized (neat to 1:10)
Lavage, sputum,
other fluids Sample diluent HB To 0.5% final* User optimized (neat to 1:10)
User optimized (at least 1:2 for
Lysate Sample diluent HB To 0.5% final* 50 to 500 µg/ml final protein)
* At least 0.5% final BSA is recommended to stabilize analytes and reduce adsorption to labware.