Bio-Rad Bio-Plex Manager™ MP Software Upgrade - Manual (Page 33)

Optimizing the Standard Curve in Bio-Plex Manager 6.x
Getting Started Guide | 31
3. Review the optimization report and identify any analytes for which there are
comments or that have not been successfully optimized.
It will be difficult to generate meaningful results if many of your unknowns do
not fall within the usable range of the curve, that is, between the lower limit of
quantitation (LLOQ) and the upper limit of quantitation (ULOQ). Values between
the LLOQ and background fluorescence and values above the ULOQ can be
interpreted qualitatively.