Bio-Rad Whole Gel Eluter and Mini Whole Gel Eluter - Manual (Page 7)

Lid
The lid covers the electrical contacts during elution to provide electrical insulation. The
lid cannot be removed without breaking the electrical circuit. It can be placed on the eluter in
only one alignment, so that the anode and cathode connections cannot be accidentally reversed.
Cutting Template
The cutting template is a guide for excising the portion of the slab gel intended to be elut-
ed. It also functions as a template for cutting your own upper and lower filter paper and cel-
lophane membrane.
Ruler
The ruler has been calibrated to the distance between elution channels. It can be used as
a guide to approximate where individually resolved protein bands may elute.
In order to purify an individual protein, the nearest contaminant must be at least 5 mm
away on a preparative gel for the Mini Whole Gel Eluter or 6 mm for the Whole Gel Eluter.
2.2 Vacuum Harvester Components
* The Mini Whole Gel Eluter uses 14, 1.5 ml microcentrifuge tubes.
5
Vacuum tubing
Harvest needle
array 30, 12 x 75 mm
tubes
*
Harvesting lid
Vacuum control
valve
Harvesting
tube rack