Bio-Rad Whole Gel Eluter and Mini Whole Gel Eluter - Manual (Page 10)

4. Insert the elution chamber core, aligning the holes for the screws and the electrode stem.
Finger tighten the hex screws in numerical order (crisscross pattern) while push-
ing down on the core. (See diagram below.)
Finger tighten the hex screws in numerical order (criss-cross pattern) while pushing down
on the core.
5. Turn the assembly upside down over a sink and shake out any excess buffer squeezed
into the buffer overflow channel during the tightening of the screws.
6. Fill the elution chamber with elution buffer so as to cover all of the channels. Carefully
remove any bubbles trapped in or around the channels.
7. Lay the preparative gel on a clean flat surface and place the cutting template on top of it.
Use the template to outline the portion of the gel to be eluted. The template is made of clear
acrylic and has numbered lines with which to align the template with the dye front and
molecular markers. Wear clean gloves when handling the gel.
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