Bio-Rad Model 225 Tube Gel Casting Stand - Manual (Page 2)

Table of Contents
Page
Section 1 Introduction......................................................................................1
Section 2 Equipment and Reagents...............................................................1
2.1 Equipment ....................................................................................................1
2.2
Description of Major Parts............................................................................2
2.3 Additional Required Equipment ...................................................................3
2.4 Accessories for the Mini Electrophoresis System........................................3
Section 3 Solutions ..........................................................................................4
3.1 Solutions for the First-Dimension.................................................................4
3.2 Preparation of First-Dimension Tube Gels ..................................................5
3.3 Solutions for the Second-Dimension ...........................................................5
Section 4 Safety Instructions ..........................................................................6
Section 5 The First-Dimension (IEF) ..............................................................7
5.1 First-Dimension Tube Gels ..........................................................................7
5.2 Casting Tube Gels .......................................................................................7
Section 6 Running the First-Dimension (IEF) ...............................................9
6.1 Electrode Preparation ..................................................................................9
6.2 Sample Preparation ...................................................................................10
6.3 First-Dimension Sample Loading...............................................................10
6.4 Power Conditions .......................................................................................10
Section 7 The Second-Dimension ................................................................11
7.1 Preparation of the Stacking Gel .................................................................11
7.2 Removing the Gel from the Tube and Loading onto the Slab Gel ............11
7.3 Running the Slab Gels ...............................................................................12
Section 8 Maintenance of Equipment ..........................................................12
Section 9 Troubleshooting ............................................................................12
Appendix Electrophoresis Chemicals..........................................................15