Bio-Rad Model 225 Tube Gel Casting Stand - Manual (Page 15)

7. To equilibrate the tube gel, overlay it with enough SDS sample buffer to cover it
completely, (about 200 µl), and allow it to sit for up to 10 minutes. After
equilibration, overlay with running buffer. Fill the electrode buffer reservoirs,
and begin the second-dimension run. An agarose overlay is recommended, but
is not necessary in this procedure.
7.3 Running the Slab Gels
Instructions for casting and running mini slab gels are in the Mini-PROTEAN cell
instruction manual. Suggestions for staining and destaining the slab gels are also in
the Mini-PROTEAN cell manual.
Section 8
Maintenance of Equipment
Mini-PROTEAN tube adaptor Rinse thoroughly with distilled water after
every use.
Sample reservoirs After use, rinse with laboratory detergent
Flexible tubing connectors solution, then rinse with distilled water.
Casting tube
Mini-PROTEAN electrophoresis cell Refer to Mini-PROTEAN cell instruction
manual.
Section 9
Troubleshooting Guide
1. No load or no current detected by a. Be sure that the IEF run includes at
the power supply. IEF gel runs least 8 tubes, even if some of the tubes
with high voltage and low current. are blank (contain no sample). Make
Typically, an IEF run will end with suret he blank tubes contain
a current reading at or below ampholytes.
1 mAmp.
b. Check that no air bubbles are trapped
at the top or bottom of the tube.
c. Check the limitations of the power supply.
Some power supplies will register an
error message when the current goes
below 1–10 mAmps because they
cannot detect low current.
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