Bio-Rad High-Throughput Dodeca™ Gel Stainers - Manual (Page 19)

Section 6
Maintenance and Chemical Compatibility
Maintenance
To clean the components of the Dodeca stainer, follow the instructions below.
The Dodeca stainer components are not dishwasher compatible.
Cleaning the Solution Tank, Staining Trays, and Shaking Rack
After each run, wash the solution tank, staining trays, and shaking rack
thoroughly with a non-scratch pad and mild detergent (Bio-Rad Cleaning
Concentrate diluted to the recommended working concentration, catalog #161-0722)
followed by a complete rinse with distilled water.
Note: For optimum results with fluorescent and silver staining, it is essential to
maintain clean components. These stains are more susceptible to contaminants in
the solution tank than Coomassie based stains. Residual Coomassie Brilliant Blue
quenches the fluorescent signal. Protein or metallic contaminants will catalyze non-
specific formation of metallic silver. Users may choose to utilize separate Dodeca
stainers for each stain used in the laboratory.
If more stringent cleaning is needed, use the reagents listed below. We
recommend performing a 5 minute rinse followed by a thorough rinse with distilled
water. Avoid exposing the stainer to these chemicals for a prolonged amount of
time.
• 2% Bleach (0.1% Sodium hypochlorite)
• 70% Ethanol
• 20% Hydrogen peroxide
• 50% w/v (7.8M) Nitric acid
Cleaning the Lid
First, remove the shaker motor and the shaker control unit from the lid. Then,
wash using a non-scratching pad and a mild detergent followed by a complete
rinse with distilled water.
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