Bio-Rad High-Throughput Dodeca™ Gel Stainers - Manual (Page 18)

5.2 SYPRO Ruby Protein Gel Stain
No. of
Step Solution(s) Time Cycles
1. Fix 40% Methanol 30 minutes–overnight 1
10% Acetic acid
2. Stain SYPRO Ruby 3 hours to overnight 1
3. Rinse* 10% Methanol 30–60 minutes 1
7% Acetic acid
4. Rinse H
2
O 30–60 minutes 1
* This rinse step is recommended to minimize background fluorescence and to prevent excessive gel swelling.
5.3 Dodeca Silver Stain Kit
The Dodeca Silver Stain kit is specifically formulated for ease-of-use with the
Dodeca stainer. This silver stain kit reduces clean up time by minimizing silver
deposits on the Dodeca stainer (compared to the Bio-Rad Silver Stain kit). In
addition, stained protein samples are amenable to mass spectrometry protein
identification analysis without further sample modification. See the Dodeca Silver
Stain kit instruction manual (part #411-0150) for detailed instructions on how to
prepare solutions.
Note: A minimum of four gels is recommended when silver staining using the
Dodeca stainers.
No. of
Step Solution(s) Time Cycles
1. Fixing 40% Ethanol 30 min–overnight 1
10% Acetic acid
2. Sensitizing Sensitizer Concentrate 30 min 1
Background Reducer Concentrate
3. Rinsing H
2
O 10 min 3
4. Staining Silver Reagent Concentrate 20–30 min 1
5. Rinsing H
2
O 1 min 1
6. Developing Development Buffer Concentrate 10–30 min (variable) 1
Background Reducer Concentrate
Image Developer Concentrate
7. Stop/ Storage 5% Acetic Acid 10 min–overnight 1
8. Rinsing H
2
O 10 minutes 1
15