Bio-Rad Criterion Dodeca Cell - Manual (Page 8)

Table 4. Running Conditions (for 12 Criterion precast gels, 1.0
mm thickness)
Gel and Buffer Voltage Initial Current Final Current Run Time
System (approximate) (approximate) (approximate)
Tris-HCl 200 V 1.0–1.4 A 400–500 mA 56 minutes
Tris/Glycine/SDS
or Tris/Glycine
The PowerPac 200 power supply will be limited at maximum power of 200W for a few minutes
(5–10 minutes) at this time the voltage will read less than the indicated voltage setting (about 180 V).
The Bromophenol Blue dye was run to the bottom of the gel.
Tris-Tricine 125 V 1.6 A 850 mA 110 minutes
Tris/Tricine/SDS
The PowerPac 200 power supply will be limited at maximum power of 200W for a few minutes
(20 minutes) at this time the voltage will read less than the indicated voltage setting (about 115 V).
TBE 200 V 500 mA 400 mA 76 minutes
Tris/Boric
Acid/EDTA
The gels were run until the first dye (Bromophenol Blue) cleared the gel.
TBE-Urea 200 V 500 mA 250 mA 76 minutes
Tris/Boric
Acid/EDTA
The gels were run until the second dye (Xylene Cyanole) was at the very bottom of the gel, the first
dye (Bromophenol Blue) was run off the gel
Note: Precast and Tris-Tricine gels will run slightly warmer then handcast, Tris-HCl and
TBE gels. Precast gels are prepared with Tris-HCl pH 8.6 and most handcast gels are cast
using Tris-HCl pH 8.8, this minor variation will alter the run times slightly, precast gels will
run a little faster.
2.4 Opening Criterion Cassettes and Removing the Gel
a. After electrophoresis is complete, turn off the power supply and disconnect the electrical
leads.
b. Remove the cell from the stir plate. Be sure to pick up the tank from the bottom. Remove
the lid from the tank.
c. Remove the gel cassette. Pour off and discard the buffer from the Integral Buffer Chamber.
Note: If the lower buffer will be reused, do not pour the depleted buffer from the integral
upper buffer chamber into the tank. The lower buffer can be reused up to 10 times depending
on usage (see guidelines in Section 3).
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