Bio-Rad Criterion Dodeca Cell - Manual (Page 7)

d. Load samples with a Hamilton syringe or a pipette with gel loading tips. Alternatively, use
the sample loading guide to align the pipet tip with the sample wells.
Note: If sample diffusion is of concern during loading, 2 or 3 gels may be loaded outside
the tank and then transferred into the tank to be run under very low voltage (10–20 volts).
Then the next 2 or 3 gels may be loaded and inserted into the tank and run at 10–20 volts. This
process is continued until all gels are loaded and ready to run. Buffer levels in both the upper
and lower chambers should be checked to insure they are appropriate.
e. Fill tank to the Fill Level line indicated on the tank. See Table 1 for total buffer volumes.
Tank sitting on stir plate
f. Place the lid on the tank, aligning the color-coded banana plugs and jacks. It is easiest to
align the side with the banana plugs and drain cover first and then slide the lid down in
to place.
Placing lid on tank
g. Set the power supply to recommended conditions and start the run. See Table 4 for
recommended running conditions.
5
Stir bar
Align this side first,
then slide down to fit.