Bio-Rad Criterion™ Cell - Manual (Page 7)

2.2 Power Conditions
1. Place the lid on the tank. Make sure to align the color-coded banana plugs and jacks
according to color. A stop on the lid prevents incorrect orientation.
2. Insert the electrical leads into a suitable power supply with the proper polarity.
Apply power to the Criterion cell and begin electrophoresis; 200 volts constant is
recommended for SDS-PAGE and most native gel applications. Run time is approximately
50–55 minutes at 200 volts for SDS-PAGE. Please see Criterion Gel instruction manual for
more detailed running conditions of these and other Criterion gel types.
2.3 Gel Removal
1. After electrophoresis is complete, turn off the power supply and disconnect the electrical
leads.
2. Remove the lid and carefully lift out the Criterion gel cassette. Pour off and discard the
running buffer.
3. Repeat for second Criterion gel cassette if two gels were run.
4a. Use the cassette-opening tool built into the lid to break the weld-joint on the Criterion
gel cassette(s). Place the Criterion gel cassette's upper buffer chamber over the built-in
opening wedge of the lid. Push the cassette straight down until the upper edge of the
upper buffer chamber contacts the top of the lid and the weld-joint at the top of the
cassette is broken. Pull the two cassette halves apart.
Fig. 6. Opening the Criterion Gel cassette after electrophoresis.
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