Bio-Rad Criterion™ Cell - Manual (Page 6)

2.1 Sample Loading
1. Load the samples into the wells with a Hamilton syringe or a pipette using gel loading tips.
2. Use Bio-Rad’s patented sample loading guides to locate the sample wells. Insert the
Hamilton syringe or pipette tip into the slots of the guide and fill the corresponding wells.
Fig. 4. Outlined sample wells and sample loading guides take the guesswork out of samples
loading.
Note: Load samples slowly to allow them to settle evenly on the bottom of the well. Be
careful not to puncture the bottom of the well with the syringe needle or pipette tip.
3. Fill the tank to the line molded into the sides of the tank or the lower edge of the gels
upper buffer chamber (approximately 800 ml). It is important to fill the tank to the
proper level to prevent overheating during running. If the tank is over-filled, buffer
will/may overflow onto the bench.
Fig. 5. Filling the Criterion Cell tank with buffer.
4