UVP Life Science - Manual (Page 173)

Perform Area Density Analysis
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Grey Level calculations are used when the sample is imaged using reflective light. Grey level of an area
is simply the sum of grey levels of all pixels in the area. Changes are reflected on the curve after the
window is closed.
Types of Calibration Curves
Two types of curves options can be defined using the Intensity Calibration Curve button:
• Standard Optical Density - Use this type if the sample is excited through transmitted light (such
as the FirstLight uniform UV Illuminator by UVP). Also useful when imaging colorimetric samples.
• Free Form - Use this type when fine-tuning input and output intensity values or when imaging
fluorescent/luminescent samples to produce results in Grey Levels.
Apply Pre-Defined Intensity Calibration Curve
• To apply a pre-defined intensity calibration curve to a previously defined region of interest, click
the Area Density Action Tab > Calibration menu button > Intensity Calibration.
• An Intensity Calibration Curve window opens. To select a previously defined intensity curve,
click the down arrow next to Edit List in the upper right hand corner of the window. A list of
intensity curve options will appear.
• Select the appropriate curve from the list. (For example: choose Black Band Intensity Curve if
black bands are captured in the image)
• Based on the user selection, Free Form or Std. Optical Density will be automatically selected.
• Click OK.
Add New Intensity Calibration Curve
• Click onto Edit List in the Intensity Calibration Curve window.
• A Point List Collection window will appear. Click onto Add to add a new curve. Name the curve,
then select OK.
• The new user-created curve will appear in the drop down menu list. This new curve can be
deleted and edited in the Point List Collection window, if desired.
• With the new curve selected, choose Free Form or Std. Optical Density.