Leica Biosystems M80 Measuring User Manual
Page 7
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Measuring – User Manual
̈ Place stage micrometer on
the table insert and set
sharpness.
̈ Select the magnification
with which you will later
want to take measurements.
̈ Set eyepiece graticule and
lens micrometer to be free of
parallax (see page 4).
̈ Align stage micrometer (X) close to and in parallel
with the eyepiece graticule (Y).
̈ Focus both pitches down to a single point.
In the example: 0 and 10
̈ Count: The number of mm (inches) of the stage
micrometer (X) corresponds to a certain number of
intervals on the eyepiece graticule (Y).
̈ In the example:
7,8mm on the stage micrometer corresponds to 120
intervals on the eyepiece graticule
̈ Calculate calibration value using calibration formula
and note down.
X stage micrometer: number of mm (inches)
Y eyepiece graticule: number of intervals
Calibrating
Calibration formula
x
0
1
2
3
4
5
6
7
8
9
10
11
12
1
2
3
4
5
6
7
8
10
y
X
Y
mm (inch) = calibration value mm (inch)