C.B.S. Scientific TTGEK-2401-220 - Manual (Page 52)

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SECTION 4
Running Conditions, con’t.
4.3 Recommended Running Conditions, TTGE
Sample PAA% % Denaturant
(UF/l)
Temp Ramp
(°C/hour)
Time
(hours)
Buffer Volts
K-ras exon 1 (5) 10 29 (7M UF/l) 1.7°C
(63°-68°)
3 1x TAE 130
PTEN (6) 10 29 (7M UF/l) 2ºC
(46º-58º)
6 1x TAE 130
p53 (7) 8 29 (7M UF/l) 3ºC
(56º-66º)
3.3
(mini-gel)
1.25x TAE 130
ß-thalassemia (7) 6 24 (6M UF/l) 2ºC
(56º-66º)
4.5 1.25x TAE 130
CF Exon 7 (11) 6 24 (6M UF/l) 2ºC
(50º-60º)
5 1.25x TAE 130
CF Exon 10, 11 (8) 6 24 (6M UF/l) 2ºC
(50º-60º)
5 1.25x TAE 130
mtDNA (10) 6 24 (6M UF/I) 1.2ºC
(53º-64º)
6-7 1.25x TAE 145
Nitrosospira-
Bacterial (9)
6 29 (7M UF/l) 1ºC
(40º-55º)
16 1.25x TAE 100