C.B.S. Scientific TTGEK-2401-220 - Manual (Page 43)

TTGE Instruction Manual, version 8/30/2011
43
www.cbsscientific.com
3.8 Preparation of the Cassettes
1. After polymerization rinse gel plate assembly with D.I. water to remove excess acrylamide or
denaturants from plate exterior.
2. Remove comb and quickly transfer gel sandwich to cassette by removing all #2 clamps.
LEAVE GEL WRAP IN PLACE. The Gel Wrap acts as a barrier and prevents perpendicular
electrical fields from interfering with outside lanes. Re-clamp to cassette using #1 clamps/ 2
per side. Dislodge the entire bottom portion of the Gel Wrap by pulling from the plates to
allow buffer contact with the gel. (Fig 3-10)
3. To avoid capturing a bubble under gel, immerse cassette at an angle into pre-heated buffer
tank (Fig.3-7). If a bubble is captured or forms under the gel, expel using a buffer-filled syringe
fitted with a bent 16 gauge needle. Attach buffer recirculating tubing to each upper reservoir
(Fig. 3.8 & Fig 3-9). Rinse each well with buffer to remove non-polymerized acrylamide or
excess urea and fill upper reservoir with buffer.
Fig. 3-7
dislodge Gel Wrap™ all the way
across bottom of glass plates