C.B.S. Scientific TTGEK-2401-220 - Manual (Page 35)

TTGE Instruction Manual, version 8/30/2011
35
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3.4 Running the Heater/Stirrer
1) Fill reservoir with at least 23 Liters of buffer. (refer to section 3.1 for working buffer volume).
2) Turn Heater/Stirrer “On” (Fig 3-3). Reference section 3.2l Setting the Bath Temperature Set Point (page
15)
a) For DGGE, set at 60ºC.
b) For TGGE, select temperature ramp program, see section 4.3.
Fig. 3-3
3.5 Preparation/Cleaning of Glass Plate for Gel Casting
Hand wash both plates with a high quality lab detergent followed by a complete rinsing with dH
2
O. Air dry or use a
lint-free tissue. Spray/wipe the chosen inner surfaces of the plate set with 95% ethanol and dry with lint-free tissue.