C.B.S. Scientific TTGE-2401 - Manual (Page 7)

www.cbsscientific.com 7
HB90001
4. Place frame #1 onto clamping base,
so that frame is flush with clamping
platform as shown at right.
5. Place one piece of wet cellophane sheet evenly onto platform.
6. Place up to 4 ClearPAGE™ gels onto cellophane making sure all gels are
inside frame perimeter.
B) Instructions for Gel Drying stained gels using the HB90025 air drying frame:
1. After de-staining, rinse the stained gel once in distilled water (100ml/gel) for 5 minutes.
2. Equilibrate the rinsed gel in ClearPAGE drying solution (HB04510) or water containing 5% glycerol
(25ml/.gel) for 10 minutes at room temperature, rocking.
3. Soak two pieces of cellophane (HB02520) in distilled water.
GEL DRYING
Gels may be dried without cracking between cellophane after equilibrating with
ClearPAGE™ Drying Solution (HB04510) or with 5% glycerol in water (25ml per
gel, rock for 30 minutes). No alcohol is required.
A) Instructions for Gel Drying stained gels using the HB90001 air drying
frame:
1. After de-staining, rinse the stained gel once in distilled water (100ml/gel) for
5 minutes.
2. Equilibrate the rinsed gel in ClearPAGE drying solution (HB04510) or water
containing 5% glycerol (50ml/gel) for 10 minutes at room temperature.
3. Soak two pieces of cellophane (HB02025) in distilled water.
4. Place the solid frame on 1 or 2 pipette tip boxes.
5. Place one piece of wet cellophane evenly on top, followed by the gel (s), a
second piece of wet cellophane, and finally the open frame. (Alternatively,
use two open frames for faster gel drying).
6. Carefully smooth away any air bubbles between the cellophane sheets.
7. Clip the frames together, and place on lab bench. Allow to air dry
horizontally overnight.
8. When the gel is completely dried, remove the frame and cut away excess
cellophane for storage.