C.B.S. Scientific TTGE-2401 - Manual (Page 6)

ClearPAGE Precast Gel Running Instructions 7-1-14 6
GENERAL NOTES ON USING COOMASSIE STAINS
One may use either the heated or ambient protocol at any step, but the first step works best when heated as
it fixes the proteins better. For the sharpest bands and fastest results, C.B.S. Scientific recommends using
the heated protocols. Notes on heating solutions in a microwave:
1) Do not heat gel in microwave for temperatures above 70ºC, as solution may get too hot and dissolve the
agarose.
2) For 10% or 20% Ethanol solutions, heat 50ml for 30 seconds on high (600W). The water portion may be
heated separately, and alcohol added after heating.
3) For water, heat 100ml for 1 minute on high (600W) and then add to the gel.
4) Microwave ovens may vary. Check temperature results for your oven and adjust times.
d) Staining with Silver-Stain Solutions
For ammoniacal silver staining protocols (e.g., Pierce Silver SNAP™ or Invitrogen SilverXpress™
Silver Stain kits), fix proteins for 10 minutes with 200ml per gel of 50% methanol/10% acetic acid/20mM
sodium bisulfite. For the bisulfite, use Cat. No. LB30001 Antioxidant: 1ml per 200ml fixative. For
the SilverXpress kit, use the Tris Glycine protocol using the above fixative, then follow the remaining
protocol. For the Silver SNAP kit, use the Tris Glycine protocol using the above fixative, then follow then
follow the remaining protocol.