C.B.S. Scientific TTGE-2401 - Manual (Page 11)

www.cbsscientific.com 11
Tris-Glycine-SDS Transfer Buffer, 10X (FB82500)
Ingredient MW Molarity Qty/Liter
Tris (free base) 121.14 0.25M 30.3 g
SDS (2%) 288.38 - 20.0 g
Glycine 75.07 1.92M 144.1 g
Ultrapure water (fill to) - - 1000 ml
* pH should be between 8.4 and 8.6 at 25° C.
Standard DNA/Native Running Buffer, 20X (GB61500)
Ingredient MW Molarity Qty/Liter
Tricine (free acid) 179.17 0.8M 143.4 g
Tris (free base) 121.14 1.2M 145.2 g
Ultrapure water (fill to) - - 1000ml
* pH should be between 8.35 and 8.45 at 25° C.
DNA/Native Sample Buffer, 4x (GB33002)
Ingredient MW Molarity Qty/Liter
Glycerol (40%) - - 400 g
Ficoll-400 (4%) - - 40 g
Triethanol amine, pH7.6 149.2 0.8M 120.0
6 N HCL 36.46 - 93.0 g
EDTA Di-Sodium 372.2 2mM 7.44 g
Brilliant Blue G250
(0.025%)
- - 0.25g
Phenol Red - - 0.25 g
Ultrapure water (fill to) - - 1000 ml
* pH should be 7.6 at 25° C.