C.B.S. Scientific TTGE-2401 - Manual (Page 12)

ClearPAGE Precast Gel Running Instructions 7-1-14 12
ClearPAGE TWO-COLOR SDS MARKER
An orange/blue prestained protein marker is also available for SDS PAGE. The recombinant proteins
range in size from 7.6 kDa to 195 kDa. The orange markers are 28 kDa and 71 kDa, the other eight
bands are blue. ClearPAGE Two-Color SDS Marker is sold ready to use in a 1x LDS sample buffer
with 600μl per vial (catalog #HM05160). The purity and band sharpness mean the markers can be
used for visualizing the separation while running the gel, checking effectiveness on your blot, or as a
routine marker in place of other unstained markers.
Recommended volumes to load are:
Application 12-well gels (4.2mm wide) 17-well gels (2.2mm wide)
& Marker well on 2D gels
load volume loads/vial load volume loads/vial
See during run 10μl 60 5μl 120
See on blot membrane 4μl 150 2μl 300
See with stain 4μl 150 2μl 300
Comparison of Marker on ClearPAGE 12%, 12 well SDS gel with or with Instant Blue stain.
Molecular weights are the apparent weights in the ClearPAGE SDS gel system.
195
142
96
71
48
33
28
22
12
7.6
Instant Blue
Stained
Natural kDa